DIGITALNA ARHIVA ŠUMARSKOG LISTA
prilagođeno pretraživanje po punom tekstu



ŠUMARSKI LIST 5-6/2000 str. 12     <-- 12 -->        PDF

M. Id/ojtic: SASTAV ETERIČNIH ULJA IZ IGLICA... Šumarski list hr. 5-6, CXXIV (20(H)), 263,-270


methyl silicone and Carbowax 20 M phases. J. Van den Dool, H. & P. D. Kratz, 1963: Ageneraliza-


Chromatogr. 503: 1-24. tion of the retention index system including line-


H i ltunen, R. & I. Laakso, 1995: Gaschromatogra- ar temperature programmed gas-liquid partition


phic analysis and biogenetic relationships of mo- chromatography J. Chromatogr. 11: 463-471.


noterpene enantiomers in Scots pine and juniper Von Rudioff, E., 1975: Volatile leaf oil analysis in


needle oils. Flavour Fragr. J. 10: 203-210. chemosystematic studies of North American co-


Kubeczka,K. H. & W. Schultze, 1987: Biology nifers. Biochem. Syst. Ecol. 2: 131-167.


and chemistry of conifer oils. Flavour Fragr. J. 2: Yazdani, R., J. E. Nilsson & T. Ericsson, 1985:


137-148. Geographical variation in the relative proportion


Orav, A., T. Kailas & M. Liiv, 1996: Analysis of of monoterpenes in cortical oleoresin of Pinus


terpenoic composition of conifer needle oils by sylvestris in Sweden. Silvae Genet. 34 (6):


steam destillation/extraction, gas chromatogra- 201-208.
phy and gas chromatography - mass spectrometry.
Chromatographia 43 (3/4): 215-220.


ZAHVALA - Acknowledgement


Ovaj je rad rađen u okviru projekta "Istraživanje va- mi je stavio na raspolaganje biljke zasađene na poku-


rijabilnosti različitih familija međuvrsnih hibrida dvo- snim plohama u Đurđevačkim peskima i u Arboretumu


igličavih borova" financiranog od Javnog poduzeća Lisičine. Također zahvaljujem dr. HartwiguW. Pfe i f-


"Hrvatske šume" p.o. Zagreb i projekta "Hibridi četiri- hoferu s Instituta za fiziologiju bilja Sveučilišta u


ju vrsta borova i njihovo određivanje" financiranog od Grazu, koji mije stavio na raspolaganje opremu za rad


Ministarstva znanosti i tehnologije Republike Hrvat- na ovim istraživanjima,
ske. Zahvaljujem prof. dr. sc. Želimiru Borzanu što


SUMMARY: The composition of needle volatiles of Scots pine (Pinus sylvestris
L. = sy), European black pine (P. nigra Arnold = ni), Japanese red pine (P. densi-
flora Sieb, et Zucc. = de) and Japanese black pine (P. thunbergiana Franco = th)
was investigated with gas chromatography (GC) and gas chromatography/mass
spectrometry (GC/MS).


The number of detected components differs for individual species (European
black pine 122, Scots pine 116, Japanese red pine 122, Japanese blackpine 87). A
total of 55 components was identified. The identified components accounted for
90.1 % w/w sample of Scots pine, 94.4 % w/w sample of European black pine,
81.3 % w/w sample of Japanese red pine and 94.9 % w/w sample of Japanese
black pine. All identified components were terpenes, except tmns-2-hexenal,
which is n-alkene. The amount of monoterpenes was the highest (sy 54.3 %, ni
64.9 %, de 61.7 %, th 77.3 %), followed by sesquiterpenes (sy 35.5 %, ni 27.1 %,
de 13.6 %, th 16.4 %) andditerpenes (sy 0.2 %, ni2.3 %, de 5.9%, th 0.73 %).


In terms of the composition of needle essential oil, P. sylvestris, P. nigra, P. den-
siflora and P. thunbergiana do not differ qualitatively, but their quantitative difference
is considerable. The components that are specific for Scots pine are
A´-carene and endo- 1-bourbonanol. Germacrene D is characteristic for European
blackpine, thunbergol for Japanese red pine, and ß-pinene for Japanese black
pine. These components occur in a significantly higher amount in essential oils of
the mentioned species than in those of other analysed species.


Of all the components present in the needle essential oils, the amount ofa-pi-
nene is the highest in Scots pine, European blackpine and Japanese red pine (sy
32.9 %, ni 42.7 %, de 25.8 %), while that of ß-pinene is the highest in Japanese
red pine (34.1 %).


It has been shown with cluster analysis that Scots pine and Japanese red pine
are the most similar in terms of needle essential oil composition. European black
pine is similar to them, while Japanese black pine differs considerably from the
other three analysed species.


Key words : Pinus sylvestris!., P. nigra Arnold, P. dens´iüora Sieb, et Zucc,
P. thunbergiana Franco, essential oils, terpenes, GC, GC/MS, cluster analysis.


270