DIGITALNA ARHIVA ŠUMARSKOG LISTA
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| ŠUMARSKI LIST 11-12/2011 str. 21 <-- 21 --> PDF |
O. Tančeva Crmarić, S. Štambuk, Z. Šatović, D. Kajba: GENOTIPSKARAZNOLIKOST DIVLJE TREŠNJE ... Šumarski list br. 11–12, CXXXV (2011), 543-555 SUMMARY: Wild cherry (Prunus aviumL.) has recently drawn great attention because of its noble and high quality wood, but also because of its importance in preserving genetic diversity. Clonal material used to investigate genotypic diversity of the wild cherry was taken from the clonal seed orchard (Kutina Forest Office) and consisted of 24 selected plus trees from the area of north-western Croatia. The clones were analyzed by 15 selected microsatellite markers (SSR), chosen by the ECPGR. A wealth of allelic variations was found in SSR loci, while a high degree of polymorphism confirmed the existence not only of extensive morphological but also a very significant genetic diversity. Based on pairwise proportion of shared allele distance (DPSAM) among the 24 clones of wild cherry, the average genetic distance of 0.573 was calculated. The smallest genetic distance (DPSAM= 0.100) was recorded between the genotypes ‘KP2’ and ‘KP5’ (Kloštar Podravski, region Koprivnica), which coincided in 27 out of 30 alleles, whereas the largest genetic distance (DPSAM= 0.933) was found between the genotypes ‘Đu2’ (Đulovac, Bjelovar region) and ‘L3’ (Lipovljani, Zagreb region), which differed in 28 out of 30 alleles. The genetic distance matrix, based on pairwise proportion of shared allele distance (DPSAM), did not show a clear classification of wild cherry individuals with regard to their origin, i.e. region (Koprivnica, Bjelovar, Zagreb). The analysis of molecular variance (AMOVA) revealed a significantly higher percentage (95.88 %) of the total microsatellite diversity caused by the differences among the invidividuals within the regions, compared to that caused by the differences between the studied regions (4.12 %). The . – statistics, amounting to 0.041, was highly significant (P < 0.01) and indicates the existence of specific regional structurality of genetic diversity. It is presented by the axes of factorial correspondence analysis (FCA). The first axis explains 63.76 % of the total inertia and discriminates the individuals from the Zagreb region from those from the Bjelovar and Koprivnica regions, while the second axis with 36.24 % discriminates the individuals from the Bjelovar region from those in Koprivnica region. Key words:Prunus aviumL., microsatelites SSR, genetic diversity |