DIGITALNA ARHIVA ŠUMARSKOG LISTA
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| ŠUMARSKI LIST 5-6/2012 str. 37 <-- 37 --> PDF |
Implications for the use of forest reproductive material of common ash (fraxinus excelsior L .) in Slovenia based on the analysis of nuclear microsatellites Prijedlozi za upoTREbu šumskog reprodukcijskog materijala običnog jasena (Fraxinus excelsior L.) u Sloveniji na bazi analize jezgrinih mikrosatelita Marjana Westergren1, Kristjan Jarni2, Robert Brus3, Hojka Kraigher4 Summary Assumption that forest reproductive material is better adapted to local conditions is the basis of current forest policy that promotes the use of local material. Genetic diversity and structure of two approved seed stands and three non-approved stands of Fraxinus excelsior L. were analysed with nuclear microsatellites to get genetically based support for the use of its reproductive material in Slovenia. Genetic diversity was high (HE = 0.80) and differentiation between populations measured as FST (FST = 0.018) low to nonexistent when measured with genetic distances. Calculated allelic indices for seed stands were the same or a bit above the Slovenian average with two exceptions. Based on the analysis of five microsatellite loci, no restrictions for transferring forest reproductive material within the studied range can be presented. However, collection of forest reproductive material from seed stand Rodik should follow good seed collection practices to ensure high genetic diversity of reproductive material. Key words: Fraxinus excelsior, common ash, Slovenia, microsatellites, genetic diversity, genetic structure, forest reproductive material Introduction Uvod Common ash (Fraxinus excelsior L.) is the most wide-spread and commercially important of the three ash species in Slovenia. It grows from lowlands to the forest border, even though it is rarely observed at elevations above 1 000 m (Brus 2008). It is a highly outcrossing wind pollinated species with complex polygamous breeding system. Trees with male, female and hermaphroditic flowers are observed (FRAXIGEN 2005). Although self-fertilisation in common 1 Dr Marjana Westergren, Slovenian Forestry Institute, Večna pot 2, SI-1000 Ljubljana, Slovenia, marjana.pucko@gozdis.si 2 Mag Kristjan Jarni, Department of Forestry and Renewable Forest Resources, Biotechnical faculty, University of Ljubljana, Večna pot 83, SI-1000 Ljubljana, Slovenia, kristjan.jarni@bf.uni-lj.si 3 Doc dr Robert Brus, Department of Forestry and Renewable Forest Resources, Biotechnical faculty, University of Ljubljana, Večna pot 83, SI-1000 Ljubljana, Slovenia, robert.brus@bf.uni-lj.si 4 Prof dr Hojka Kraigher, Slovenian Forestry Institute, Večna pot 2, SI-1000 Ljubljana, Slovenia, hojka.kraigher@gozdis.si |