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ŠUMARSKI LIST 5-6/2012 str. 40     <-- 40 -->        PDF

locally common alleles, i.e. alleles with frequency equal to or higher than 0.05, were found in one population only. Locally common alleles that were shared among at least two populations, were observed in all five populations.
Analysed allele indices for seed stands were slightly above the Slovenian average (mean values based on all five analysed populations), except for the number of private alleles in seed stand Grofija and the observed number of alleles in seed stand Rodik.
Mean observed heterozygosity (HO = 0.72) was lower than the expected heterozigosity (HE = 0.80), causing a significant positive mean inbreeding coefficient (FIS = 0.102, P = 0.000). Overall inbreeding coefficient decreased to 0.058 after omitting locus FEMSATL16 from the analysis, which showed substantial deficiency of heterozygotes (results not shown), but remained significantly different from zero (P = 0.000).
Genetic structure – Genetska struktura
Differentiation between common ash populations based on allele size (RST = –0.007) was not significantly higher than differentiation based on allele identity (FST = 0.018; P = 0.993). The null hypothesis that stepwise mutations do not contribute to genetic differentiation could not be rejected even for individual loci. Therefore only information based on allele identity (FST) was considered further.
Population Razpotje was significantly different from all other populations based on pair-wise FST values, which were between 0.023 and 0.040. Except from additional differentiation between populations Grofija and Soteska, other pairs of populations did not differ among each other (Table 3).
In contrast to pair-wise FST values, which take into account two populations at a time, distance based methods use all the data simultaneously. The latter failed to detect differentiation between populations. Bootstrap values were low, never exceeding 50 %, and branch lengths short (Figure 2).
Differentiation between populations measured as FST / (1 – FST) did not increase significantly with the natural logarithm of geographical distance between common ash populations (P = 0.330). Therefore no isolation by distance among the analysed populations can be reported.
the overall pattern of genetic diversity observed at microsatellite loci of common ash was comparable to that seen in other common ash studies in Europe. Mean genetic diversity in the five studied populations was high (HE = 0.80) and comparable to that obtained in other common ash studies where HE ranged between 0.73 and 0.82 (Ballian et al. 2008, Ferrazzini et al. 2007, Heuertz et al. 2004, Heuertz et al. 2003, Heuertz et al. 2001, Sutherland et al. 2010). On the other hand, differentiation between populations was low. Null alleles did not substantially affect the estimation of