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We researched the influence of irrigation and micro-relief in nurseries on morphological properties of pedunculate and sessile oak seedlings (2+0). The test plots were established in the forest nursery Hajderovac, Forestry Office Kutjevo, Forest Administration Branch Požega. The sowing of sessile oak acorns was carried out from 7 to 11 October 2005, and of pedunculate oak on 14 October 2005. Irrigation was taken as a treatment for the pedunculate oak seedlings (1 non-irrigated, 2 irrigated) while for the sessile oak seedlings it was the micro-relief (1 on slopes, 2 on flat terrain). At the end of the second growing season a random sample of seedlings was taken from the test plots for a morphological analysis. The morphological analysis of the washed root system was performed on the scanner Epson Expression 10000XL while the WinRhizo pro.V.2005 software was used to determine five root variables: length (cm), perimeter (cm2), average diameter (mm), volume (cm3) and number of root tips (pieces). For the seedlings of pedunculate oak a variance difference higher than the set limit (p=0,05) was established for the following variables: root length (p=0,293703), root perimeter (p=0,21999), root volume (p=0,281090) and the number of root tips (p=0,829142). Mann Whitney’s U-test established a statistically significant difference in height (p=0,000000), root collar diameter (p=0,000284) and the average root diameter (p=0,011632) between the non-irrigated (1) and the irrigated (2) seedlings of pedunculate oak. With an average height of 578 mm (322–790 mm) the irrigated pedunculate oak seedlings (2+0) were by 305 mm higher compared to the non-irrigated ones. The average root collar diameter of the irrigated seedlings was also by 0,92 mm larger compared to the non-irrigated ones and amounted to 6,91 mm (4,93–9,90 mm). On average the root diameter of the irrigated seedlings was by 0,12 mm larger compared to the non-irrigated ones. The irrigated seedlings of pedunculate oak also showed a larger total root length (cm), root perimeter (cm2) and number of root tips (pcs) with respect to the non-irrigated ones but the mentioned characteristics are not significant. For the seedlings of sessile oak a variance difference higher than the set limit (p=0,05) was established for the following variables: height (p=0,178360), root collar diameter (p=0,077099) and root length (p=0,148820). Mann Whitney’s U-test established a statistically significant difference in all the four tested variables (root perimeter (p=0,000010), average root diameter (p=0,025060), root volume