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ŠUMARSKI LIST 7-8/2016 str. 53     <-- 53 -->        PDF

et al., 1998), antithrombotic (Arslan et al., 2011), antifungal (Orhan et al., 2007), antinociceptive (Bor et al., 2012) effects and that they are effective in early periods of congestive heart failure (Rietbrock, 2001).
Hawthorn refers to deciduous trees or shrubs belong to genus Crataegus L. of family Rosaceae which generally has thorns (Christensen, 1992; Dönmez, 2004). Genus hawthorn has around 200 species around the world. Although more than 1000 names have been reported for this plant around the world, it has 100-200 species. Some characteristics of the genus such as leaf and seed morphology, number of seed and fruit color are polymorphous. Polymorphism and hybridization are the reason for high number of synonyms. There are 26 hawthorn taxa growing in Turkey (Dönmez, 2007).
This study aims to determine chemical contents of different hawthorn species which are a wild species distributed in Western Anatolia.
Material of the study consisted of Crataegus samples collected from A1-A2, A3,B1-B2, B3, C2 and C3 squares according to squaring system in Flora of Turkey (Davis et. al., 1988) in 2010-2014 period (Figure 1, Table 1). Field study was conducted in the study area in blooming (May-June), fruit ripening (September-October) periods of Crataegus species. Samples were collected and registered and plant samples were maintained at Forest Botanic Laboratory of Süleyman Demirel University, Faculty of Forestry for drying and storage according to herbarium techniques. Plants were identified and kept at Süleyman Demirel University, Faculty of Forestry herbarium.
Determination of volatile components – Određivanje hlapljivih komponenti
Volatile components of hawthorn leaves and flowers were determined according to solid phase microextarction method (Vichy et. al., 2003). Collected plant materials were dried at room temperature. 2 gr of each sample were weighted and placed in glass bottles and were heated at 60 C0 for 15 minutes. Then they were soaked in injector bottle with a suitable fiber tip and absorbed for 30 min. The compounds that were exposed to fiber tip were injected to injection block of GC unit and were kept for 5 min for absorption.
GC-MS device (Shimadzu QP 5050, Japan) was used to determine volatile components. HP-5 MS (30 m x 0.25 mm long and 0.25 um film thickness) column and Helium (10 psi flow rate) was used as carrier gas in the device. Injection block temperature was 240 °C and detector temperature was 250 °C.
Determination of fruit seed fixed oil composition – Određivanje sastava nehlapljivih ulja u sjemenkama voća
GC-MS (with FID Detector, Shimadzu QP 5050, and Japan) in Suleyman Demirel University Experimental and Observational Student Research and Application Center was used