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In this paper we investigated potential of portable chlorophyll meter CCM-200 (Opti-Sciences, Tyngsboro, MA, USA) to estimate total nitrogen concentration in different types of Pedunculate oak leaves. Research was conducted on 30 plants grown in field trial, during two vegetation periods (in years 2009 and 2010). Fertilization treatment (carried out in early spring in 2009) and meteorological conditions caused differences between investigated years regarding third-flush shoots (that can be considered as potential trigger for translocation of nitrogen in leaves and branches of investigated plants). Eight calibration curves was constructed based on spring and second-flush leaves, sampled in July and September in 2009 and 2010. These equations shown to be are reliable (p < 0,001) in describing relationship between chlorophyll content index, measured with chlorophyll meter, and total nitrogen concentration in spring and second-flush leaves, regarding moments of sampling (July and September 2009 and 2010). Influence of leaf flush type on homogeneity of equation parameters (i.e. slope and intercept), regarding moment of sampling, was tested using ANCOVA. Leaf flush type (spring or second-flush) did not have significant influence on slope, but did influenced intercepts in three moments of sampling (July 2009, and July and September 2010). Homogeneity of equation parameters was observed only for one moment of sampling, e.g. September in 2009, when third-flush shoots occurred which resulted in levelling of total nitrogen concentration in sampled leaves.
Key words: Chlorophyll content index, leaf third-flush, leaf nitrogen dynamic, the parameters of the calibration equations