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ŠUMARSKI LIST 11-12/2016 str. 17     <-- 17 -->        PDF

accordance with the ISTA rules. The seeds of Narrow-leaved ash (crop 2013) in the nursery Brestje was placed under stratification in the open on 16 January 2015 and sown on 21 March 2015. Stratification lasted for 64 days. The delayed germination seeds of Narrow-leaved ash were collected on 20 October 2015. Viability was assessed using the same methodology as in the case of fresh seeds. Fresh seeds have viability of 91%. There was 9% of non-viable seed, of which the largest percent have empty seeds (5%), followed by insect-damaged seeds (3%) and seeds in which the embryo and endosperm had unstained areas or necrosis. Delayed germination seeds had seed vigour of 87%. There were 13% of non-viable seeds, of which the highest percentage were the seeds containing embryos with unstained surfaces or necroses, and fully coloured endosperm (10%). Seeds in which the embryo and endosperm had unstained surfaces or necrosis accounted for 3%. Necrosis or embryo rot, which then spread to the endosperm, was detected in non-viable delayed germination seed. As for necrosis or embryo rot, seeds were observed with complete necrosis, necrosis of cotyledons, hypocotyls and plumule, hypocotyls and radicle and radicle alone. In the spring of 2015 nursery seed germination in the nursery Brestje was 0% despite 82% vitality. A statistically significant difference was found between the viability of fresh and delayed germination seeds. The viability of delayed germination seeds was 4% lower than that of fresh seeds. Based on these studies, the collection of seeds of Narrow-leaved ash is recommended to begin from the second half of August, since such seeds manifest high viability. When sowing seeds in the nursery, account should be taken of its age, pre-sowing preparation and sowing time. A recommendation for future nursery production of Narrow-leaved ash seedlings with a generative method involves determining the optimal type and duration of seed stratification with respect to micro-relief growth diversity of parent trees from which the seeds are collected (micro-depressions, unsoaked micro-depressions, micro-elevations), climate and seed zoning.
KEY WORDS: seed viability, the tetrazole method, Narrow-leaved ash, ISTA rules, seed dormancy