DIGITALNA ARHIVA ŠUMARSKOG LISTA
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ŠUMARSKI LIST 1-2/2018 str. 23     <-- 23 -->        PDF

In this study, we combined descriptive and multivariate statistical methods using the morphological leaf traits and environmental features to examine the diversity of grey alder populations distributed across the Croatian continental and Alpine-Dinaric biogeographical regions. Our main objectives were: 1) to reveal the levels of population variation and divergence within and among natural populations of A. incana; and 2) to test correlations between geographic, environmental and morphological variation.
Material and methods
Materijal i metode
Plant material and leaf characters studied – Biljni materijal i istraživane značajke lista
The material for morphometric analysis was collected in July 2016 in seven natural populations of A. incana in Croatia (Figure 1). The study encompassed four populations from the Alpine-Dinaric region (P01 - Gerovo, P02 – Turke, P03 – Čedanj; P04 – Lukovdol), and three populations from the continental region (P05 – Varaždin, P06 – Slatina; P07 – Donji Miholjac). Each of the studied populations was represented by 20 trees and each tree by 20 healthy and undamaged leaves of short shoots from the external, sunlit part of the crown. The minimum distance between individual trees was at least 50 m, to avoid the sampling of close relatives. Vouchers for the populations studied were deposited in the herbarium at the Faculty of Forestry of the University of Zagreb (DEND).
The leaves were scanned and measured using the WinFolia program (WinFolia TM2001). A total of nine characteristics were measured (Figure 2). The accuracy of measurements was 0.1 mm, and the following morphological characteristics