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ŠUMARSKI LIST 5-6/2018 str. 30     <-- 30 -->        PDF

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The concentration of photosynthetic pigments (chlorophylls and carotenoids) in leaves of forest trees is a good indicator of the photosynthetic efficiency, level of nutrition with nitrogen and autumn leaf senescence. Thus the photosynthetic pigment concentrations is one of the most significant parameters related to the physiological status of plant. Change in pigment concentrations of leaves indicate presence of environmental stress. Well-timed detection of environmental stress play a crucial role in preventing damage on seedlings and saplings in forest nurseries and wood mass production in forest cultures and plantations.
Traditional methods for determining the concentrations of photosynthetic pigments represent an obstacle to continuous monitoring concentrations of photosynthetic pigment in leaves of forest trees, primarily because they are relatively expensive, long lasting and require destruction of plant material. However, portable optical chlorophyll meters such as CCM-200 (Opti-Sciences, Tyngsboro, Massachusetts, USA) and SPAD-502 (Minolta Camera Co., Osaka, Japan) is a reliable alternative to traditional laboratory methods. To use chlorophyll meters as a tool for estimating photosynthetic pigment concentrations in leaves, it is necessary to construct regression equations (calibration equations) that reliably describe the relationships between the relative index of the total chlorophyll content in leaves (CCI) and the total pigment concentrations in leaves, determined by standard laboratory methods.
In the present study, we set up experiment to establish the ability of the hand-held CCM-200 chlorophyll meter to accurately estimate the content of the photosynthetic pigments in leaves of Quercus robur L. with contrast level of soil moisture. The objectives of the present work were: (i) determine whether the long-lasting drought period significantly influences the relationship between the CCI obtined by CCM-200 and the concentration of photosynthetic pigments (total chlorophyll and carotenoid); (ii) construct calibration equations for non-destructive estimation of photosynthetic pigments in Q. robur leaves; (iii) to analyse the improvement estimation of photosynthetic pigments concentration when CCI was corrected with specific leaf mass.
The research was carried out during the year 2015 on 60 two-years old plants grown in the greenhouse located in the Croatian Forestry Institute Jastrebarsko. Plant material was grown in 50 liter containers