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ŠUMARSKI LIST 5-6/2018 str. 37     <-- 37 -->        PDF

cerris, Q. suber, Q. crenata and Q. × viridis (Figure 3a). Another plastid haplotype was shared between Q. robur and Q. petraea individuals while Q. ilex, Q. coccifera and Q. pubescens displayed different, unique haplotypes. The haplotype network showed that the five haplotypes are arranged according to three lineages, each representing the three main infrageneric groups encompassing the here studied oaks: Group Cerris (Q. cerris, Q. suber, Q. crenata), Group Ilex (Q. ilex and Q. coccifera) and Group Quercus (Q. robur, Q. petraea and Q. pubescens). Interestingly, Q. × viridis individuals were all included in the only haplotype of the Cerris group, diverging from the Q. ilex haplotype by 27 mutational steps (Figure 3a).
The nuclear dataset was characterized by 11 ITS2 + 5.8S ribotypes (Figure 3b) and higher nucleotide diversity π = 0.01617 (Table 2). In contrast to the obtained plastid haplotypes, the majority of the included Quercus taxa as well as some individuals of the same species showed private ITS2 + 5.8S sequence variants. In total, nine ribotypes occurring in single individuals were detected. Two variants were shared among different taxa: one between Q. suber, Q. crenata and Q. × viridis (from Rijeka) and the other between Q. crenata and Q. × viridis (from Zadar) (Figure 3b). Three ribotypes were detected in Q. cerris and two in Q. suber and in Q. × viridis. Similar to the cpDNA network, lineages corresponding to the three infrageneric groups could be recognized. Ribotypes found in the Q. × viridis individuals were included in the Cerris lineage, separated from the ribotype displayed by Q. ilex by at least 10 mutational steps.
The reconstructed MP phylogenetic relationships of the nuclear ribosomal sequences revealed three main clades, still corresponding to the three Quercus infrageneric groups (Figure 4). Within the Cerris clade, the putative Q. × viridis individual from Islam Latinski (Zadar) and the other putative Q. × viridis individual from Donje Jelenje (Rijeka), formed a subclade together with Q. suber and Q. crenata individuals from Croatia, Slovenia and Italy, with no or very low resolution, and separated from the Q. cerris sequences. In particular, Q. × viridis individual from Zadar (Croatia) was placed as a sister taxon next to the sampled Q. crenata from the Latium region in Italy (Figure 4). Separation of the Q. × viridis individuals from those belonging to the