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ŠUMARSKI LIST 3-4/2021 str. 44     <-- 44 -->        PDF

ash seeds (Table 1). Weevil and moth larvae found in narrow-leaved ash seeds were identified based on the morphological and molecular genetic analysis, as L. enucleator and Pseudargyrotoza conwagana (Fabricius, 1775, Lepidoptera: Tortricidae), with 98.21% and 99.06% sequence similarity to reference sequences in NCBI GenBank, respectively. Wasp larvae and adults found in this ash species were not successfully identified (Figure 3).
Morphological and molecular genetic analysis of wasps in damaged seeds of green ash revealed that found pupae belong to species Eupelmus urozonus (Dalman, 1820, Hymenoptera: Eupelmidae), and larvae to family Pteromalidae (Hymenoptera), with 99.35% and 93.30% sequence similarity to reference sequences in NCBI GenBank, respectively. Wasp adults remained unidentified. Weevil larvae were identified as L. bischoffi, combining their morphological characteristics according to Gosik et al. (2017) and molecular genetic analysis, because the obtained sequence was ambiguous and shared only 89.53% similarity with other L. bischoffi sequences deposited in NCBI GenBank. Moth larvae was identified based on its morphological characteristics as P. conwagana. Fly larvae were not successfully identified (Figure 3).
Relatively small number of damaged and empty narrow-leaved ash seeds was found in this research, implying their good health status regarding insect infestation. Although seeds of introduced green ash revealed higher insect occupation, especially those collected in the urban area, most of the specimens found (81%) were parasitoids, indicating that pest populations are naturally held under control. In total, four insect taxa were identified to species level in this research, of which three can be categorised as seed pests (L.