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ŠUMARSKI LIST 9-10/2021 str. 63     <-- 63 -->        PDF

measurements were taken three times a day (morning, noon and evening) with a thermometer until completion of the study. Monthly tempe­ratures determined in Greenhouse-3 medium are given in ­Figure 1.
Within the scope of the study, Indole-3-Butyric Acid (IBA) and α-Naphthalene Acetic Acid (NAA) were selected among auxin group hormones in order to encourage the rooting of the cuttings. The phytohormones (PH) prepared in 1000 and 5000 ppm doses were applied to the cuttings in each greenhouse medium (GM) and rooting medium (RM). At this stage, while the cuttings were transferred to the rooting medium, the bottom of the cuttings prepared with a length of 10-12 cm were dipped in powdered hormone prepared in the determined doses. The study was set up to be three replications using “randomised complete block design”. For each taxon (Chamaecyparis lawsoniana ‘Ellwoodii’, Cryptomeria japonica ‘Elegans’ and x Cupressocyparis leylandii), a total of 900 cuttings were planted to rooting including 720 treatment cuttings (1 taxon x 2 hormones x 2 doses x 3 greenhouse medium x 2 rooting medium x 10 cuttings x 3 replications) and 180 control cuttings (1 taxon x 3 greenhouse medium x 2 rooting medium x 10 cuttings x 3 replications). In addition, on the planted cuttings, the first callus and first root formation dates, rooting percentage (RP), callus percentage (CP), root length (RL) and the number of roots (RN) were determined.
Statistical analysis: Variance analysis and Duncan test were conducted on the obtained data using IBM SPSS 23 statistical program. Analysis of variance (univariate) was used to determine the effects of different greenhouse medium, rooting medium and hormones on measured parameters. Additionally, Duncan test was made to determine the groups that were found in terms of hormones and greenhouse medium for RP, CP, RL and RN.
3. RESULTS
3. REZULTATI
Chamaecyparis lawsoniana ‘Ellwoodii’ cuttings at the end of 106 days, Cryptomeria japonica ‘Elegans’ cuttings at the end of 109 days and x Cupressocyparis leylandii cuttings at the end of 213 days were removed from the whole rooting medium. After 25 days from planting for C. lawsoniana ‘Ellwoodii’, the first callus formations were determined in IBA 1000, IBA 5000, NAA 1000 and NAA 5000 ppm treatments in perlite rooting medium at GM-2, and also in control, NAA 1000 and NAA 5000 ppm treatments in peat rooting medium at GM-2. The first root formations (after 35 days from planting) of C. lawsoniana ‘Ellwoodii’ occurred in IBA 1000, IBA 5000 ppm treatments in peat rooting medium at GM-2. For C. japonica ‘Elegans’, it is determined that the first callus formation occurred at the end of 22 days in IBA 1000, IBA 5000, NAA 1000 and NAA 5000 ppm treatments in perlite rooting medium at GM-2, and the first root formation occurred in IBA 5000 and NAA 5000 ppm treatments after 34 days from planting in the same greenhouse and rooting medium. For x Cupressocyparis leylandii, the first callus formation occurred at the end of 29 days in perlite rooting medium at GM-1 (NAA 1000 and NAA 5000 ppm) and GM-2 (IBA 5000 ppm), and in peat rooting medium at GM-3 (NAA 1000 and NAA 5000 ppm). The first root formation occurred at the end of 36 days in NAA 5000 ppm treatment in perlite rooting medium at GM-2. On the rooted cuttings of studied taxa, rooting percentage, callus percentage, root length and the number of roots are given in Table 1 and Table 2 depending on the effects of the different greenhouse medium, rooting medium and hormones.
The highest rooting and callus percentages obtained in different greenhouse, rooting medium and hormones differ depending on the taxa studied. Accordingly, the highest