DIGITALNA ARHIVA ŠUMARSKOG LISTA
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Each trial unit used 50 seeds and trials were repeated four times. Therefore, a total of 10 trial units × 50 seeds × 4 replicates = 2000 seeds tested. During the 28 days, i.e., the total germination period, the seeds were monitored daily for deterioration and fungal growth, and distilled water was used to meet the water requirement of the seeds. In the experiment, the seeds that had rootlets extending to the size of the seed were considered germinated. The germination rate indicates the ability of seeds to germinate quickly. The germination rate of the germinated seeds was recorded on the 7th, 10th, and 14th days (Saatçioğlu 1971). However, if some types of seeds such as red pine are not subjected to pre-treatment such as stratification, etc., germination may not occur in seven days (Çetin 2010). In this study, since germination started after seven days, the germination rate was calculated from the germination data on the 10th and 14th days, and the overall germination percentage at the end of the 28th day. Germination rate and germination percentage were calculated from the seeds germinated using the following formula: GP GP (%): Germination percentage ni: The number of germinated seeds on day i N: The total number of tested seeds Statistical analyses – Statističke analize A two-way interaction test was run for the seed germination rates for three periods (10, 14, and 28 days). Altitude zones were at two elevation levels (L=low and H= high) and the cone age at five levels (3, 4, 5, 6, and 7 years). Since the interactions between altitude zone and cone age were significant on seed germination for all the tree periods (p = 0.005), the cone-age data were merged with data of the corresponding altitude zones and the Tukey mean separation analysis was conducted on the combined data. The SAS (1996) package program was used in the analysis. The results were considered different at the α = 0.05 level. RESULTS REZULTATI The first germination in the seeds was on the 8th day. As seen in Table 1, the fastest and highest germination among all sample areas was realized in the seeds obtained from the 3-year-old (control) cones in the 0-200 m lower altitude zone. Germination at 10, 14, and 28 days was 20.5%, 62.5%, and 89.1%, respectively. The difference in the germination rate between seeds of different ages gradually decreased towards the end of the germination period (28 days) (Table 1; Figs 1, 2, and 3). Seed germination rates of less than 20% were recorded for all cone types in the first 10-day period of the experiment. In this period, the highest germination was in L3 and this value was 95% higher than L5. The germination rate in the lower altitude zone was higher than that in the higher altitude zone. The lowest value in the entire trial was in H7, which was about half of the average germination rate of both altitude zones (Fig. 1). Germination rates had increased 2 - 3 times by the 14th day of the experiment. In this period, the germination rate of L3 was 34% higher than that of L7, and L3 had also a 67% higher germination rate compared to that of the average of the |