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Summary The application of newer remote sensing methods, such as aerial and terrestrial lidar scanning and the use of "Structure-from-motion" (SfM) photogrammetry, complemented field data collection and enabled 3D mapping of forest fuel layers, greatly simplifying and improving their characterization. However, these methods are not suitable for quantifying forest floor characteristics. For this purpose, it is still necessary to collect data using classical field methods, determining the presence of subhorizons and their depth, while the characteristics of the forest floor: bulk density, load, carbon concentration and carbon stock are determined in the laboratory. Therefore, it is still common practice to create regression equations that allow operatives to determine the amount of available forest floor fuel and the carbon stock it contains based on the depth of the forest floor, which is an easily measurable variable, or to determine forest floor loading by subhorizon and overall. Forest floor information is used in models for predicting forest fire behavior and spread, in fire effects models, in planning and monitoring mechanical fuel reduction, in quantifying fuel consumption and smoke emissions, in quantifying carbon stocks, in describing habitat and its productivity, and in planning for preparedness. As stands of holm oak (Quercus ilex L.) and pubescent oak (Quercus pubescens Willd.) are located in the Mediterranean part of Croatia, where the risk of forest fires is the highest, and the previously published data on the forest floor are not suitable for the models, the main objectives of the research were to determine the depth, bulk density and load of individual subhorizons of the forest floor and to create regression equations that allow estimating the amount of available fuel in the |